TLR4 Protein (AA 214-291) (GST tag)

Catalog No. ABIN1322876

Product Details

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Protein Type: Recombinant

Protein Characteristics: AA 214-291

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This TLR4 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Purpose: TLR4 (Human) Recombinant Protein (Q01)

Sequence: PMNFIQPGAF KEIRLHKLTL RNNFDSLNVM KTCIQGLAGL EVHRLVLGEF RNEGNLEKFD KSALEGLCNL TIEEFRLA

Characteristics: Human TLR4 partial ORF ( NP_612564, 214 a.a. - 291 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Hayakawa, Pham, Seo, Miyamoto, Maki, Terasaki, Sakadžić, Boas, van Leyen, Waeber, Kim, Arai, Lo: "CD200 restrains macrophage attack on oligodendrocyte precursors via toll-like receptor 4 downregulation." in: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, (2015) (PubMed).

Hendriks, Hua, Chabot: "Analysis of mechanistic pathway models in drug discovery: p38 pathway." in: Biotechnology progress, Vol. 24, Issue 1, pp. 96-109, (2008) (PubMed).

Target Details

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4 (TLR4 Products)

Synonyms: ARMD10 Protein, CD284 Protein, TLR-4 Protein, TOLL Protein, Lps Protein, Ly87 Protein, Ran/M1 Protein, Rasl2-8 Protein, toll like receptor 4 Protein, toll-like receptor 4 Protein, TLR4 Protein, Tlr4 Protein

Background: Full Gene Name: toll-like receptor 4
Synonyms: ARMD10,CD284,TOLL,hToll

Gene ID: 7099

NCBI Accession: NM_138554

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins