TNF alpha Protein (AA 18-111, Extracellular Domain) (DYKDDDDK Tag)
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- Target See all TNF alpha Proteins
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 18-111, Extracellular Domain
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Origin
- Human, Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TNF alpha protein is labelled with DYKDDDDK Tag.
- Application
- SDS-PAGE (SDS)
- Brand
- MegaTNF-alpha™
- Specificity
- Binds to human and mouse TNF-R1 and TNF-R2.
- Cross-Reactivity
- Human, Mouse (Murine)
- Characteristics
- The extracellular domain of human TNF-alpha (aa 85-233) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.02EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product TNF alpha Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg, WEHI 164 cells).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Lot specific
- Buffer
- Lyophilized. Contains PBS.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - Expiry Date
- 6 months
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- Target
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Alternative Name
- MegaTNF-alpha (TNF alpha Products)
- Synonyms
- DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein
- Background
- Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. MultimericTNF-alpha™ is a high activity construct in which two trimeric TNF-alpha ligands are artificially linked via the collagen domain of ACRP30. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-alpha, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-alpha (TNF-alpha (human) (multimeric) (rec.) . Since the MultimericTNF-alpha™ mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-alpha protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-alpha or MultimericTNF-alpha™ can be used.
- Molecular Weight
- ~34kDa (SDS-PAGE)
- UniProt
- P01375
- Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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