GM-CSF Protein (His tag)

Catalog No. ABIN1589599

Product Details

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This GM-CSF protein is labelled with His tag.

Sequence: APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQETRH HHHHH

Characteristics: Length (AA): 135
Chromosomal location: 5q31.1
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells [Kitamura T et al, J Cell Physiol, 1989]. The ED50 for this effect is typically < 0.1 ng/mL corresponding to a specific activity of ≥ 1 x 107 units/mg.

Purity: > 98 % by SDS-PAGE. Visualized by silver stain

Endotoxin Level: < 0.1 ng per µg of GM-CSF

Application Details

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized rh GM-CSF is soluble in water and most aqueous buffers and can be reconstituted in water to a concentration of 0.1 mg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.

Buffer: PBS

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: The lyophilized powder although stable at room temperature for 3 weeks, is best stored desiccated at -20 °C. Reconstituted GM-CSF should be stored in working aliquots at -20 °C. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).

Expiry Date: 3 weeks at RT

Target Details

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF (CSF2 Products)

Synonyms: GMCSF Protein, GM-CSF Protein, CSF2 Protein, Csfgm Protein, Gm-CSf Protein, MGI-IGM Protein, Gm-csf Protein, Gmcsf Protein, CSF Protein, colony stimulating factor 2 Protein, colony stimulating factor 2 (granulocyte-macrophage) Protein, CSF2 Protein, Csf2 Protein

Background: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), a 14,6 kDa protein consisting of 127 amino acid residues (Ala18-Glu144) and fused to a C-terminal His-tag (6x His), is a potent species specific stimulator of bone marrow cells and several other cell types. GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity receptors required for human GM-CSF signal transduction have been shown to be heterodimers consisting of a GM-CSF-specific α chain and a common β chain that is shared by the high-affinity receptors for IL-3 and IL-5.
Synonyms: CSF2, GMCSF

Molecular Weight: 15-18 kDa

NCBI Accession: NP_000749, NM_000758

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin