FLIP Protein (AA 1-376) (His tag)

Catalog No. ABIN1612831

Recombinant FLIP protein expressed in Yeast.

Quick Overview for FLIP Protein (AA 1-376) (His tag) (ABIN1612831)

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Protein Type: Recombinant

Origin: Orang-Utan

Source: Yeast

Application: ELISA

Purity: > 90 %

Product Details

Protein Characteristics: AA 1-376

Purification tag / Conjugate: This FLIP protein is labelled with His tag.

Sequence: MSAEVIHQVE EALDTDEKEM LLFSCRDVAI DVVPPNVRDL LDILRERGKL SVGDLAELLY RVRRFDLLKR ILKMDRKAVE THLLRNPHLV SDYRVLMAEI GEDLDKSDVS SLIFLMKDYM GRGKISKEKS FLDLVVELEK LNLVAPDQLD LLEKCLKNIH RIDLKTKIQK YKQSVQGAGT SYRNVLQAAI QKSFKDPSNN FRLHNGRSKE QRLKEQLGTQ QEPVKKSIQE SEAFLPQSVP EERYKMKSKP LGICLIIDCI GNETELLRDT FTSLGYEVQK FLHLSMHGIS QILGQFACMP EHRDYDSFVC VLVSRGGSQS VYGVDQTHSG LPLHHIRRMF MGDSCPYLAG KPKIFFIQNY VVSEGQLEDS SLLEVD

Specificity: Pongo abelii (Sumatran orangutan)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Background: Recommended name: CASP8 and FADD-like apoptosis regulator Cleaved into the following 2 chains: 1.
CASP8 and FADD-like apoptosis regulator subunit p43 2.
CASP8 and FADD-like apoptosis regulator subunit p12

UniProt: Q5RD56

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development