GCDH Protein (AA 45-438) (His tag)
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- Target See all GCDH Proteins
- GCDH (Glutaryl-CoA Dehydrogenase (GCDH))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 45-438
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GCDH protein is labelled with His tag.
- Purpose
- Recombinant Human Glutaryl-CoA Dehydrogenase Mitochondrial/GCDH (N-6His)
- Sequence
- MNHKVHHHHH HMRPEFDWQD PLVLEEQLTT DEILIRDTFR TYCQERLMPR ILLANRNEVF HREIISEMGE LGVLGPTIKG YGCAGVSSVA YGLLARELER VDSGYRSAMS VQSSLVMHPI YAYGSEEQRQ KYLPQLAKGE LLGCFGLTEP NSGSDPSSME TRAHYNSSNK SYTLNGTKTW ITNSPMADLF VVWARCEDGC IRGFLLEKGM RGLSAPRIQG KFSLRASATG MIIMDGVEVP EENVLPGASS LGGPFGCLNN ARYGIAWGVL GASEFCLHTA RQYALDRMQF GVPLARNQLI QKKLADMLTE ITLGLHACLQ LGRLKDQDKA APEMVSLLKR NNCGKALDIA RQARDMLGGN GISDEYHVIR HAMNLEAVNT YEGTHDIHAL ILGRAITGIQ AFTASK
- Characteristics
- Recombinant Human Glutaryl-CoA Dehydrogenase Mitochondrial/GCDH is produced with our E. coli expression system. The target protein is expressed with sequence (Arg45-Lys438) of Human GCDH fused with a His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product GCDH Protein
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Glutaryl-CoA Dehydrogenase (GCDH) (AA 1-438) protein (GST tag)
GCDH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glutaryl-CoA Dehydrogenase (GCDH) (AA 45-438) protein (His tag)GCDH Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Glutaryl-CoA Dehydrogenase (GCDH) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)GCDH Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- GCDH (Glutaryl-CoA Dehydrogenase (GCDH))
- Alternative Name
- GCDH (GCDH Products)
- Synonyms
- ACAD5 Protein, GCD Protein, zgc:56505 Protein, zgc:77704 Protein, 9030411L18 Protein, AI266902 Protein, D17825 Protein, glutaryl-CoA dehydrogenase Protein, glutaryl-CoA dehydrogenase a Protein, glutaryl-Coenzyme A dehydrogenase Protein, GCDH Protein, Gcdh Protein, gcdha Protein
- Sub Type
- Fusionprotein
- Background
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Glutaryl-CoA Dehydrogenase Mitochondrial (GCDH) is an enzyme that acts upon glutaryl-coenzyme A, creating crotonyl-coenzyme A. It plays a role in the metabolism of lysine, hydroxylysine and tryptophan. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive Glutaryl-CoA and electron-transfer flavoprotein to (E)-but-2-enoyl-CoA, CO2 and reduced electron-transfer flavoprotein. A defect in this enzyme is associated with neurological condition glutaric acidemia type 1 and cause a progressive form of early-onset generalized dystonia.
Alternative Names: Glutaryl-CoA Dehydrogenase Mitochondrial, GCD, GCDH - Molecular Weight
- 45 kDa
- UniProt
- Q92947
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