MGMT Protein (AA 1-207) (His tag)
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- Target See all MGMT Proteins
- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-207
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MGMT protein is labelled with His tag.
- Purpose
- Recombinant Human 6-O-Methylguanine-DNA Methyltransferase/MGMT (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPALHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
- Characteristics
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Recombinant Human 6-O-Methylguanine-DNA Methyltransferase/MGMT (N-6His)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product MGMT Protein
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O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His-IF2DI Tag)
MGMT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (GST tag)MGMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-100) protein (GST tag)MGMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
O6-Methylguanine-DNA-Methyltransferase (MGMT) protein (Myc-DYKDDDDK Tag)MGMT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mMTrisHCl,1 mM EDTA, pH 8.0 .
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
- Alternative Name
- Methylated-DNA--Protein-Cysteine Methyltransferase/MGMT (MGMT Products)
- Synonyms
- AGT Protein, AI267024 Protein, Agat Protein, O-6-methylguanine-DNA methyltransferase Protein, MGMT Protein, Mgmt Protein
- Background
- Recombinant Human Methylated-DNA--protein-cysteine methyltransferase/MGMT is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asn207) of Human MGMT fused with a 6His tag at the N-terminus.
- Molecular Weight
- 23.8 kDa
- UniProt
- P16455
- Pathways
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
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