FGF10 Protein (AA 40-208) (His tag)

Catalog No. ABIN2017802

Product Details

Target: FGF10 (Fibroblast Growth Factor 10 (FGF10))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 40-208

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This FGF10 protein is labelled with His tag.

Characteristics: ED50 < 20 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0x 10^4 units/mg.
AA 40-208, expressed with N-terminal His-Tag.

Purity: > 95 % by SDS-PAGE analysis.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Fibroblast Growth Factor-10 (rhFGF-10) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhFGF-10 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: FGF10 (Fibroblast Growth Factor 10 (FGF10))

Alternative Name: Fibroblast Growth Factor-10 (FGF-10) (FGF10 Products)

Synonyms: fgf10 Protein, fgf-10 Protein, wu:fd11d03 Protein, zgc:109774 Protein, FGF10 Protein, AEY17 Protein, BB213776 Protein, Fgf-10 Protein, Gsfaey17 Protein, FGF-10 Protein, fibroblast growth factor 10a Protein, fibroblast growth factor 10 Protein, fgf10a Protein, FGF10 Protein, fgf10 Protein, Fgf10 Protein

Background: Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares the homolog and receptor FGFR2-IIIb with KGF. However, unlike KGF which induces the proliferation and differentiation of various epithelial cells, FGF-10 is an essential factor for the budding and branching morphogenesis during the multi-organ development via the instructive mesenchymal-epithelial interactions. FGF-10 is crucial for lung and limb development, and is regulated by Shh during early development. Recombinant human Fibroblast Growth Factor-10 (rhFGF-10) with N-terminal His-tag produced in E. coli is a single non-glycosylated polypeptide chain containing 187 amino acids. A fully biologically active molecule, rhFGF-10 has a molecular mass of 21.4 kDa analyzed by reducing SDS-PAGE.
Synonyms: Fibroblast Growth Factor-10, FGFA, Keratinocyte growth factor-2

Molecular Weight: 21.4 kDa, observed by reducing SDS-PAGE.

UniProt: O15520

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Tube Formation, Positive Regulation of Response to DNA Damage Stimulus