Sonic Hedgehog Protein (SHH) (AA 25-198) (His tag)

Catalog No. ABIN2115472

Product Details

Target: Sonic Hedgehog (SHH)

Protein Type: Recombinant

Protein Characteristics: AA 25-198

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Sonic Hedgehog protein is labelled with His tag.

Purpose: Recombinant Mouse Sonic Hedgehog/SHH

Sequence: MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKITRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGG

Characteristics: Recombinant E.coil Uracil-DNA glycosylase is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Glu229) of E.coil UNG fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.4.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Sonic Hedgehog (SHH)

Alternative Name: Sonic Hedgehog/SHH (SHH Products)

Synonyms: HHG1 Protein, HLP3 Protein, HPE3 Protein, MCOPCB5 Protein, SMMCI Protein, TPT Protein, TPTPS Protein, 9530036O11Rik Protein, Dsh Protein, Hhg1 Protein, Hx Protein, Hxl3 Protein, M100081 Protein, fc83d08 Protein, shh Protein, syu Protein, vhh-1 Protein, vhh1 Protein, wu:fc83d08 Protein, Xhh Protein, hedgehog Protein, xshh Protein, SHH Protein, twh Protein, twhh Protein, sonic hedgehog Protein, sonic hedgehog a Protein, sonic hedgehog L homeolog Protein, sonic hedgehog protein A Protein, sonic hedgehog b Protein, SHH Protein, Shh Protein, shha Protein, shh.L Protein, shh Protein, shhb Protein

Background: Uracil-DNA glycosylase(UNG)belongs to the uracil-DNA glycosylase family. The human gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. After a mutation occurs, the mutagenic threat of uracil propagates through any subsequent DNA replication steps. Once unzipped, mismatched guanine and uracil pairs are separated, and DNA polymerase inserts complementary bases to form a guanine-cytosine (GC) pair in one daughter strand and an adenine-uracil (AU) pair in the other.Half of all progeny DNA derived from the mutated template inherit a shift from GC to AU at the mutation site.UDG excises uracil in both AU and GU pairs to prevent propagation of the base mismatch to downstream transcription and translation processes.
Synonyms: Uracil-DNA glycosylase,UDG,

Molecular Weight: 19.8 kDa

UniProt: Q62226

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development