TNFSF4 Protein (AA 51-183, N-Term)

Catalog No. ABIN2666855

Product Details

Target: TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 51-183, N-Term

Origin: Human

Source: HEK-293 Cells

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.1 EU per μg of protein as determine by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Human OX40L induces proliferation of PHA-activated human T cells in a dose dependent manner. The ED50 is 0.25 - 1.0 μg/mL. Results may vary depending on human donors and the dose of PHA.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))

Alternative Name: OX40L (TNFSF4 Products)

Synonyms: TNFSF4 Protein, OX40L Protein, TNFRSF4 Protein, CD134L Protein, CD252 Protein, GP34 Protein, OX-40L Protein, OX4OL Protein, TXGP1 Protein, Ath-1 Protein, Ath1 Protein, Ox40l Protein, Txgp1l Protein, gp34 Protein, TNF superfamily member 4 Protein, tumor necrosis factor (ligand) superfamily, member 4 Protein, TNFSF4 Protein, Tnfsf4 Protein

Background: OX40L is a member of the TNF superfamily of structurally related proteins that exists primarily as a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells (APCs), such as dendritic cells and activated B cells. It is also expressed on vascular endothelial cells, mast cells, and natural killer cells. OX40L signals specifically through the OX40 receptor (CD134), which is expressed predominantly on CD4+ T cells, as well as on certain activated CD8+ T cells. OX40-OX40L interaction function as a costimulatory signal that is required for a conducive interaction between antigen presenting cells and their target T cells. OX40L enhances cellular proliferation and survival of memory and effector cells, and increases expression of RANTES, IL-2, IL-3, and IFN-γ. This interaction also induces immunoglobulin secretion by activated B cells. Besides playing a role in asthma, it has been shown that plasmacytoid dendritic cells (DCs) infiltrating a melanoma microenvironment are able to promote proinflammatory Th2 and regulatory immune profiles through OX40L and ICOSL expression. This suggests that this molecule could potentially be a therapeutic target for cancer treatment.

Molecular Weight: The 154 amino acid recombinant protein has a predicted molecular mass of approximately 17.6 kDa. The protein migrates at approximately 34 kDa in DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is His.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation, Cancer Immune Checkpoints