AKR1C4 Protein (His tag)

Catalog No. ABIN2714507

Product Details

Target: AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This AKR1C4 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human AKR1C4 protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: < 0.1 EU per μg protein as determined by LAL test

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))

Alternative Name: Akr1c4 (AKR1C4 Products)

Synonyms: 3-alpha-HSD Protein, C11 Protein, CDR Protein, CHDR Protein, DD-4 Protein, DD4 Protein, HAKRA Protein, aldo-keto reductase family 1 member C4 Protein, AKR1C4 Protein

Background: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Molecular Weight: 39.3 kDa

NCBI Accession: NP_001809

Pathways: Steroid Hormone Biosynthesis