POLK Protein (Myc-DYKDDDDK Tag)
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- Target See all POLK Proteins
- POLK (Polymerase (DNA Directed) kappa (POLK))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This POLK protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human DNA polymerase kappa protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product POLK Protein
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Polymerase (DNA Directed) kappa (POLK) (Active) protein
POLK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS-PAGE (CBB staining) ELISA, WB, Func Active
Polymerase (DNA Directed) kappa (POLK) (AA 1-472) protein (GST tag)POLK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (DNA Directed) kappa (POLK) (AA 1-560) protein (His tag)POLK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Polymerase (DNA Directed) kappa (POLK) (AA 1-870) protein (Strep Tag)Crystallography grade POLK Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Polymerase (DNA Directed) kappa (POLK) (AA 1-852) protein (Strep Tag)Crystallography grade POLK Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- POLK (Polymerase (DNA Directed) kappa (POLK))
- Alternative Name
- Dna Polymerase kappa (POLK Products)
- Synonyms
- DINB1 Protein, DINP Protein, POLQ Protein, Dinb1 Protein, POLK Protein, si:ch211-254o18.3 Protein, DNA polymerase kappa Protein, polymerase (DNA directed) kappa Protein, polymerase (DNA directed), kappa Protein, polymerase (DNA directed) kappa L homeolog Protein, POLK Protein, Polk Protein, polk Protein, Tb11.01.0080 Protein, Tb11.01.0010 Protein, Tb11.01.0020 Protein, Tb11.01.0030 Protein, Tb11.01.0040 Protein, Tb11.01.0050 Protein, Tb11.12.0001 Protein, Tb11.12.0002 Protein, Tb11.12.0003 Protein, polk.L Protein, polk-1 Protein
- Background
- This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients.
- Molecular Weight
- 98.6 kDa
- NCBI Accession
- NP_057302
- Pathways
- DNA Damage Repair
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