GCK Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)
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- Target See all GCK Proteins
- GCK (Glucokinase (Hexokinase 4) (GCK))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 3
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GCK protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Glucokinase / Hexokinase-4 (transcript variant 3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product GCK Protein
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Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (GST tag)
GCK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Glucokinase (Hexokinase 4) (GCK) (AA 356-465) protein (GST tag)GCK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)GCK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Glucokinase (Hexokinase 4) (GCK) proteinGCK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (Strep Tag)Crystallography grade GCK Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (Strep Tag)Crystallography grade GCK Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- GCK (Glucokinase (Hexokinase 4) (GCK))
- Alternative Name
- Glucokinase,hexokinase-4 (GCK Products)
- Synonyms
- FGQTL3 Protein, GK Protein, GLK Protein, HHF3 Protein, HK4 Protein, HKIV Protein, HXKP Protein, LGLK Protein, MODY2 Protein, Gk Protein, Gls006 Protein, GCK Protein, BA4487 Protein, glk Protein, hk4 Protein, hhf3 Protein, hkiv Protein, hxkp Protein, gck-A Protein, mody2 Protein, glucokinase Protein, GLUKA Protein, RNGK2 Protein, glucokinase Protein, glucokinase (hexokinase 4) L homeolog Protein, glucokinase (hexokinase 4) Protein, GCK Protein, Gck Protein, glcK Protein, Bphyt_1025 Protein, Halhy_6548 Protein, gk Protein, gck.L Protein, gck Protein
- Background
- Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
- Molecular Weight
- 51.9 kDa
- NCBI Accession
- NP_277043
- Pathways
- MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
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