PCNA Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2728440

Product Details

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PCNA protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human PCNA (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: Pcna (PCNA Products)

Synonyms: PCNAR Protein, Pcna/cyclin Protein, pcna Protein, AO090003000846 Protein, 53/13 Protein, CG9193 Protein, DmPCNA Protein, Dmel\\CG9193 Protein, MUS209 Protein, Mus209 Protein, PCNA Protein, PCNA/mus209 Protein, Pcna Protein, dPCNA Protein, l(2)02448 Protein, l(2)56F Protein, l(2)56Fa Protein, l(2)s1534 Protein, cb16 Protein, fb36g03 Protein, wu:fa28e03 Protein, wu:fb36g03 Protein, pcna-A Protein, proliferating cell nuclear antigen Protein, DNA polymerase III sliding clamp Protein, Proliferating cell nuclear antigen Protein, Proliferating cell nuclear antigen-like Protein, proliferating cell nuclear antigen L homeolog Protein, proliferating cell nuclear antigen 1 Protein, PCNA Protein, Pcna Protein, pcna Protein, MMP_RS08810 Protein, ANI_1_512014 Protein, AOR_1_1500154 Protein, MGC53867 Protein, pcna.L Protein, pcna1 Protein, LOC106420145 Protein, pcn1 Protein

Background: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Molecular Weight: 28.6 kDa

NCBI Accession: NP_002583

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy