PCNA Protein (His tag)
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- Target See all PCNA Proteins
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PCNA protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human PCNA (full length, N-term HIS tag, transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PCNA Protein
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Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) protein (GST tag)
PCNA Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Proliferating Cell Nuclear Antigen (PCNA) (AA 152-261) protein (GST tag)PCNA Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) protein (Strep Tag)Crystallography grade PCNA Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) protein (Strep Tag)Crystallography grade PCNA Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Proliferating Cell Nuclear Antigen (PCNA) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PCNA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
- Alternative Name
- Pcna (PCNA Products)
- Synonyms
- PCNAR Protein, Pcna/cyclin Protein, pcna Protein, AO090003000846 Protein, 53/13 Protein, CG9193 Protein, DmPCNA Protein, Dmel\\CG9193 Protein, MUS209 Protein, Mus209 Protein, PCNA Protein, PCNA/mus209 Protein, Pcna Protein, dPCNA Protein, l(2)02448 Protein, l(2)56F Protein, l(2)56Fa Protein, l(2)s1534 Protein, cb16 Protein, fb36g03 Protein, wu:fa28e03 Protein, wu:fb36g03 Protein, pcna-A Protein, proliferating cell nuclear antigen Protein, DNA polymerase III sliding clamp Protein, Proliferating cell nuclear antigen Protein, Proliferating cell nuclear antigen-like Protein, proliferating cell nuclear antigen L homeolog Protein, proliferating cell nuclear antigen 1 Protein, PCNA Protein, Pcna Protein, pcna Protein, MMP_RS08810 Protein, ANI_1_512014 Protein, AOR_1_1500154 Protein, MGC53867 Protein, pcna.L Protein, pcna1 Protein, LOC106420145 Protein, pcn1 Protein
- Background
- The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
- Molecular Weight
- 28.6 kDa
- NCBI Accession
- NP_872590
- Pathways
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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