XPA Protein (AA 2-273) (His tag)
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- Target See all XPA Proteins
- XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-273
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This XPA protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- Sequence
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AAADGALPEA AALEQPAELP ASVRASIERK RQRALMLRQA RLAARPYSAT AAAATGGMAN VKAAPKIIDT GGGFILEEEE EEEQKIGKVV HQPGPVMEFD YVICEECGKE FMDSYLMNHF DLPTCDNCRD ADDKHKLITK TEAKQEYLLK DCDLEKREPP LKFIVKKNPH HSQWGDMKLY LKLQIVKRSL EVWGSQEALE EAKEVRQENR EKMKQKKFDK KVKELRRAVR SSVWKRETIV HQHEYGPEEN LEDDMYRKTC TMCGHELTYE KM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human XPA Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product XPA Protein
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Xeroderma Pigmentosum, Complementation Group A (XPA) (AA 1-273) protein (GST tag)
XPA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))
- Alternative Name
- XPA (XPA Products)
- Synonyms
- CG6358 Protein, DhR14 Protein, DhXPA Protein, DmXPA Protein, Dmel\\CG6358 Protein, Dxpa Protein, EG:EG0007.8 Protein, XPAC Protein, XPA_DROME Protein, Xpa Protein, dmXPA Protein, dxpa Protein, XP1 Protein, AI573865 Protein, Xpac Protein, xpac Protein, xxpa Protein, Xeroderma pigmentosum group A-like Protein, XPA, DNA damage recognition and repair factor Protein, xeroderma pigmentosum, complementation group A Protein, xeroderma pigmentosum, complementation group A L homeolog Protein, Xpac Protein, XPA Protein, Xpa Protein, xpa.L Protein
- Background
- Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. {ECO:0000269|PubMed:19197159}.
- Molecular Weight
- 32.2 kDa Including tag.
- UniProt
- P23025
- Pathways
- DNA Damage Repair
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