PPP1R13B Protein (AA 1-1090) (Strep Tag)
-
- Target See all PPP1R13B Proteins
- PPP1R13B (Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1090
-
Origin
- Human
-
Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This PPP1R13B protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
-
MMPMILTVFL SNNEQILTEV PITPETTCRD VVEFCKEPGE GSCHLAEVWR GNERPIPFDH MMYEHLQKWG PRREEVKFFL RHEDSPTENS EQGGRQTQEQ RTQRNVINVP GEKRTENGVG NPRVELTLSE LQDMAARQQQ QIENQQQMLV AKEQRLHFLK QQERRQQQSI SENEKLQKLK ERVEAQENKL KKIRAMRGQV DYSKIMNGNL SAEIERFSAM FQEKKQEVQT AILRVDQLSQ QLEDLKKGKL NGFQSYNGKL TGPAAVELKR LYQELQIRNQ LNQEQNSKLQ QQKELLNKRN MEVAMMDKRI SELRERLYGK KIQLNRVNGT SSPQSPLSTS GRVAAVGPYI QVPSAGSFPV LGDPIKPQSL SIASNAAHGR SKSANDGNWP TLKQNSSSSV KPVQVAGADW KDPSVEGSVK QGTVSSQPVP FSALGPTEKP GIEIGKVPPP IPGVGKQLPP SYGTYPSPTP LGPGSTSSLE RRKEGSLPRP SAGLPSRQRP TLLPATGSTP QPGSSQQIQQ RISVPPSPTY PPAGPPAFPA GDSKPELPLT VAIRPFLADK GSRPQSPRKG PQTVNSSSIY SMYLQQATPP KNYQPAAHSA LNKSVKAVYG KPVLPSGSTS PSPLPFLHGS LSTGTPQPQP PSESTEKEPE QDGPAAPADG STVESLPRPL SPTKLTPIVH SPLRYQSDAD LEALRRKLAN APRPLKKRSS ITEPEGPGGP NIQKLLYQRF NTLAGGMEGT PFYQPSPSQD FMGTLADVDN GNTNANGNLE ELPPAQPTAP LPAEPAPSSD ANDNELPSPE PEELICPQTT HQTAEPAEDN NNNVATVPTT EQIPSPVAEA PSPGEEQVPP APLPPASHPP ATSTNKRTNL KKPNSERTGH GLRVRFNPLA LLLDASLEGE FDLVQRIIYE VEDPSKPNDE GITPLHNAVC AGHHHIVKFL LDFGVNVNAA DSDGWTPLHC AASCNSVHLC KQLVESGAAI FASTISDIET AADKCEEMEE GYIQCSQFLY GVQEKLGVMN KGVAYALWDY EAQNSDELSF HEGDALTILR RKDESETEWW WARLGDREGY VPKNLLGLYP RIKPRQRTLA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product PPP1R13B Protein
-
-
- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
-
- Target
- PPP1R13B (Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B))
- Alternative Name
- PPP1R13B (PPP1R13B Products)
- Synonyms
- ASPP1 Protein, p53BP2-like Protein, p85 Protein, AI449786 Protein, AW545810 Protein, Tp53bp2 Protein, Trp53bp2 Protein, protein phosphatase 1 regulatory subunit 13B Protein, protein phosphatase 1, regulatory subunit 13B Protein, protein phosphatase 1 regulatory subunit 13B L homeolog Protein, protein phosphatase 1, regulatory subunit 13Bb Protein, protein phosphatase 1, regulatory (inhibitor) subunit 13B Protein, PPP1R13B Protein, Ppp1r13b Protein, ppp1r13b.L Protein, ppp1r13bb Protein, ppp1r13b Protein
- Background
- Apoptosis-stimulating of p53 protein 1 (Protein phosphatase 1 regulatory subunit 13B),FUNCTION: Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53 (PubMed:11684014, PubMed:12524540). Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. {ECO:0000269|PubMed:11684014, ECO:0000269|PubMed:12524540}.
- Molecular Weight
- 119.6 kDa
- UniProt
- Q96KQ4
- Pathways
- p53 Signaling
-