NOTCH2 Protein (AA 1699-2471) (His tag)

Catalog No. ABIN3094222

Product Details

Target: NOTCH2 (Notch 2 (NOTCH2))

Protein Type: Recombinant

Protein Characteristics: AA 1699-2471

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This NOTCH2 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: MAKRKRKHGS LWLPEGFTLR RDASNHKRRE PVGQDAVGLK NLSVQVSEAN LIGTGTSEHW VDDEGPQPKK VKAEDEALLS EEDDPIDRRP WTQQHLEAAD IRRTPSLALT PPQAEQEVDV LDVNVRGPDG CTPLMLASLR GGSSDLSDED EDAEDSSANI ITDLVYQGAS LQAQTDRTGE MALHLAARYS RADAAKRLLD AGADANAQDN MGRCPLHAAV AADAQGVFQI LIRNRVTDLD ARMNDGTTPL ILAARLAVEG MVAELINCQA DVNAVDDHGK SALHWAAAVN NVEATLLLLK NGANRDMQDN KEETPLFLAA REGSYEAAKI LLDHFANRDI TDHMDRLPRD VARDRMHHDI VRLLDEYNVT PSPPGTVLTS ALSPVICGPN RSFLSLKHTP MGKKSRRPSA KSTMPTSLPN LAKEAKDAKG SRRKKSLSEK VQLSESSVTL SPVDSLESPH TYVSDTTSSP MITSPGILQA SPNPMLATAA PPAPVHAQHA LSFSNLHEMQ PLAHGASTVL PSVSQLLSHH HIVSPGSGSA GSLSRLHPVP VPADWMNRME VNETQYNEMF GMVLAPAEGT HPGIAPQSRP PEGKHITTPR EPLPPIVTFQ LIPKGSIAQP AGAPQPQSTC PPAVAGPLPT MYQIPEMARL PSVAFPTAMM PQQDGQVAQT ILPAYHPFPA SVGKYPTPPS QHSYASSNAA ERTPSHSGHL QGEHPYLTPS PESPDQWSSS SPHSASDWSD VTTSPTPGGA GGGQRGPGTH MSEPPHNNMQ VYA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human NOTCH2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NOTCH2 (Notch 2 (NOTCH2))

Alternative Name: NOTCH2 (NOTCH2 Products)

Synonyms: AGS2 Protein, HJCYS Protein, hN2 Protein, AI853703 Protein, N2 Protein, notch6 Protein, sb:cb884 Protein, neurogenic locus notch homolog protein 2 Protein, notch 2 Protein, notch 3 Protein, LOC581186 Protein, NOTCH2 Protein, notch3 Protein, Notch2 Protein, notch2 Protein

Background: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737). {ECO:0000250|UniProtKB:O35516, ECO:0000269|PubMed:21378985, ECO:0000269|PubMed:21378989, ECO:0000269|PubMed:25985737}.

Molecular Weight: 83.9 kDa Including tag.

UniProt: Q04721

Pathways: Notch Signaling, Stem Cell Maintenance