PRKAB1 Protein (AA 2-270) (His tag)
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- Target See all PRKAB1 Proteins
- PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-270
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This PRKAB1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- Sequence
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GNTSSERAAL ERQAGHKTPR RDSSGGAKDG DRPKILMDSP EDADIFHSEE IKAPEKEEFL AWQHDLEAND KAPAQARPTV FRWTGGGKEV YLSGSFNNWS KLPLTRSQNN FVAILDLPEG EHQYKFFVDG QWTHDPSEPI VTSQLGTVNN IIQVKKTDFE VFDALMVDSQ KCSDVSELSS SPPGPYHQEP YMSKPEERFK APPILPPHLL QVILNKDTGI SCDPALLPEP NHVMLNHLYA LSIKDGVMVL SATHRYKKKY VTTLLYKPI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Prkab1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product PRKAB1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))
- Alternative Name
- Prkab1 (PRKAB1 Products)
- Synonyms
- AMPK Protein, HAMPKb Protein, 1300015D22Rik Protein, AU021155 Protein, E430008F22 Protein, MGC82489 Protein, prkab1 Protein, wu:fk93d05 Protein, wu:fw87e09 Protein, zgc:56652 Protein, zgc:76975 Protein, zgc:92228 Protein, protein kinase AMP-activated non-catalytic subunit beta 1 Protein, protein kinase, AMP-activated, beta 1 non-catalytic subunit Protein, protein kinase, AMP-activated, beta 1 non-catalytic subunit S homeolog Protein, protein kinase, AMP-activated, beta 1 non-catalytic subunit, b Protein, protein kinase, AMP-activated, beta 1 non-catalytic subunit, a Protein, PRKAB1 Protein, Prkab1 Protein, prkab1.S Protein, prkab1 Protein, prkab1b Protein, prkab1a Protein
- Background
- Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) (By similarity). {ECO:0000250}.
- Molecular Weight
- 31.1 kDa Including tag.
- UniProt
- Q9R078
- Pathways
- AMPK Signaling, Warburg Effect
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