PTK7 Protein (AA 718-1062) (His tag)
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- Target See all PTK7 Proteins
- PTK7 (PTK7 Protein tyrosine Kinase 7 (PTK7))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 718-1062
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This PTK7 protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequence
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CKKRCKAKRL QKQPEGEEPE MECLNGGPLQ NGQPSAEIQE EVALTSLGSG PPATNKRHSA GDRMHFPRAS LQPITTLGKS EFGEVFLAKA QGVEEGATET LVLVKSLQSR DEQQQLDFRR EVEMFGKLNH ANVVRLLGLC REAEPHYMVL EYVDLGDLKQ FLRISKNKDE KLKSQPLSTK QKVALCSQVA LGMEHLSNNR FVHKDLAARN CLISAQRQVK VSALGLSKDV YNSEYYHFRQ AWVPLRWMSP EAVLEGDFST KSDVWAFGVL MWEVFTHGEM PHGGQADDEV LADLQAGKAR LPQPEGCPSK LYRLMQRCWA PNPKDRPSFS EIASTLGDSP ADSKQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Ptk7 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product PTK7 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- PTK7 (PTK7 Protein tyrosine Kinase 7 (PTK7))
- Alternative Name
- Ptk7 (PTK7 Products)
- Synonyms
- PTK7 Protein, CCK-4 Protein, CCK4 Protein, Srf Protein, 8430404F20Rik Protein, chz Protein, mPTK7/CCK4 Protein, KLG Protein, protein tyrosine kinase 7 (inactive) Protein, protein tyrosine kinase 7 Protein, PTK7 protein tyrosine kinase 7 Protein, PTK7 Protein, Ptk7 Protein
- Background
- Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis. {ECO:0000269|PubMed:15229603, ECO:0000269|PubMed:19439496, ECO:0000269|PubMed:20704721}.
- Molecular Weight
- 39.6 kDa Including tag.
- UniProt
- Q8BKG3
- Pathways
- RTK Signaling, Tube Formation
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