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ECSIT Protein (ECSIT) (AA 49-435) (His tag)

Crystallography grade ECSIT Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3130605
$11,767.31
Plus shipping costs $45.00 and $20.00 dry ice
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  • Target See all ECSIT Proteins
    ECSIT
    Protein Type
    Recombinant
    Protein Characteristics
    AA 49-435
    Origin
    Mouse
    Source
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This ECSIT protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    KDEPWLVPRP PEPQRKPIKV PAMHEDLFKP SGNRERDKAS FLNAVRSFGA HNVRKRGHVD FIYLALRKMP EFGVERDLSV YNLLLDVFPK EVFRPRNVIQ RIFVHYPRQQ ECGVAVLEQM ERHGVMPSAE TEFLLIQIFG RKSYPMLKFL RMKLWFTRFK NINPYPVPRD LPQDPLDLAK LGLRHMEPDL SAKVTVYQMS LPSDSTGMED PTQPHIVGIQ SPDQQAALAR HNPSRPVFVE GPFPLWLRNK CVYYHILRAD LPPPEEEKVE EIPEEWELYY PQKLDLEYSR SGWDDYEFDV DEVTEGPVFA MCMAGAHDQA TLIKWIQGLQ ETNPTLAQIP VVFRLARSTG ELLTTSRLEG QSPPHSPPKG PEEDDETIQA EQQQGQS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Ecsit Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    ECSIT
    Alternative Name
    Ecsit (ECSIT Products)
    Synonyms
    CG10610 Protein, DmECSIT Protein, Dmel\\CG10610 Protein, dECSIT Protein, ECSIT Protein, Sitpec Protein, SITPEC Protein, CG10610 gene product from transcript CG10610-RA Protein, ECSIT signalling integrator Protein, ecsit Protein, ECSIT signalling integrator S homeolog Protein, ECSIT Protein, CpipJ_CPIJ003637 Protein, Ecsit Protein, ecsit.S Protein
    Background
    Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development. {ECO:0000269|PubMed:10465784, ECO:0000269|PubMed:14633973}., Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase. {ECO:0000250}.
    Molecular Weight
    45.6 kDa Including tag.
    UniProt
    Q9QZH6
    Pathways
    TLR Signaling, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome
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