FANCD2 Protein (AA 1-1450) (Strep Tag)

Catalog No. ABIN3136034

Product Details

Target: FANCD2 (Fanconi Anemia, Complementation Group D2 (FANCD2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1450

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FANCD2 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequence: MISKRRRLDS EDKENLTEDA SKTMPLSKLA KKSHNSHEVE ENGSVFVKLL KASGLTLKTG ENQNQLGVDQ VIFQRKLFQA LRKHPAYPKV IEEFVNGLES YTEDSESLRN CLLSCERLQD EEASMGTFYS KSLIKLLLGI DILQPAIIKM LFEKVPQFLF ESENRDGINM ARLIINQLKW LDRIVDGKDL TAQMMQLISV APVNLQHDFI TSLPEILGDS QHANVGKELG ELLVQNTSLT VPILDVFSSL RLDPNFLSKI RQLVMGKLSS VRLEDFPVIV KFLLHSVTDT TSLEVIAELR ENLNVQQFIL PSRIQASQSK LKSKGLASSS GNQENSDKDC IVLVFDVIKS AIRYEKTISE AWFKAIERIE SAAEHKALDV VMLLIIYSTS TQTKKGVEKL LRNKIQSDCI QEQLLDSAFS THYLVLKDIC PSILLLAQTL FHSQDQRIIL FGSLLYKYAF KFFDTYCQQE VVGALVTHVC SGTEAEVDTA LDVLLELIVL NASAMRLNAA FVKGILDYLE NMSPQQIRKI FCILSTLAFS QQPGTSNHIQ DDMHLVIRKQ LSSTVFKYKL IGIIGAVTMA GIMAEDRSVP SNSSQRSANV SSEQRTQVTS LLQLVHSCTE HSPWASSLYY DEFANLIQER KLAPKTLEWV GQTIFNDFQD AFVVDFCAAP EGDFPFPVKA LYGLEEYSTQ DGIVINLLPL FYQECAKDAS RATSQESSQR SMSSLCLASH FRLLRLCVAR QHDGNLDEID GLLDCPLFLP DLEPGEKLES MSAKDRSLMC SLTFLTFNWF REVVNAFCQQ TSPEMKGKVL SRLKDLVELQ GILEKYLAVI PDYVPPFASV DLDTLDMMPR SSSAVAAKNR NKGKTGGKKQ KADSNKASCS DTLLTEDTSE CDMAPSGRSH VDKESTGKEG KTFVSLQNYR AFFRELDIEV FSILHSGLVT KFILDTEMHT EATEVVQLGP AELLFLLEDL SQKLENMLTA PFAKRICCFK NKGRQNIGFS HLHQRSVQDI VHCVVQLLTP MCNHLENIHN FFQCLGAEHL SADDKARATA QEQHTMACCY QKLLQVLHAL FAWKGFTHQS KHRLLHSALE VLSNRLKQME QDQPLEELVS QSFSYLQNFH HSVPSFQCGL YLLRLLMALL EKSAVPNQKK EKLASLAKQL LCRAWPHGEK EKNPTFNDHL HDVLYIYLEH TDNVLKAIEE ITGVGVPELV SAPKDAASST FPTLTRHTFV IFFRVMMAEL EKTVKGLQAG TAADSQQVHE EKLLYWNMAV RDFSILLNLM KVFDSYPVLH VCLKYGRRFV EAFLKQCMPL LDFSFRKHRE DVLSLLQTLQ LNTRLLHHLC GHSKIRQDTR LTKHVPLLKK SLELLVCRVK AMLVLNNCRE AFWLGTLKNR DLQGEEIISQ DPSSSESNAE DSEDGVTSHV SRNRATEDGE DEASDEQKDQ DSDESDDSSS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FANCD2 (Fanconi Anemia, Complementation Group D2 (FANCD2))

Alternative Name: Fancd2 (FANCD2 Products)

Synonyms: FANCD2 Protein, CG17269 Protein, CG31192 Protein, CG31194 Protein, Dmel\\CG17269 Protein, dmFANCD2 Protein, xfancd2 Protein, FA-D2 Protein, FA4 Protein, FACD Protein, FAD Protein, FAD2 Protein, FANCD Protein, 2410150O07Rik Protein, AU015151 Protein, BB137857 Protein, Fanconi anemia complementation group D2 Protein, CG17269 gene product from transcript CG17269-RA Protein, Fanconi anemia group D2 protein Protein, Fanconi anemia, complementation group D2 Protein, Fanconi anemia complementation group D2 L homeolog Protein, FANCD2 Protein, Fancd2 Protein, LOC100455970 Protein, fancd2 Protein, fancd2.L Protein

Background: Fanconi anemia group D2 protein homolog (Protein FACD2),FUNCTION: Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress (By similarity). Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching. {ECO:0000250, ECO:0000269|PubMed:12893777}.

Molecular Weight: 163.6 kDa

UniProt: Q80V62

Pathways: DNA Damage Repair