BLK Protein (AA 2-505) (His tag)
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- Target See all BLK Proteins
- BLK (B Lymphoid Tyrosine Kinase (BLK))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-505
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This BLK protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)
- Purpose
- Recombinant Human BLK Protein is produced by our E. coli expression system and the target gene encoding Gly2-Pro505 is expressed with a 6His tag at the C-terminus.
- Sequence
- GLVSSKKPDK EKPIKEKDKG QWSPLKVSAQ DKDAPPLPPL VVFNHLTPPP PDEHLDEDKH FVVALYDYTA MNDRDLQMLK GEKLQVLKGT GDWWLARSLV TGREGYVPSN FVARVESLEM ERWFFRSQGR KEAERQLLAP INKAGSFLIR ESETNKGAFS LSVKDVTTQG ELIKHYKIRC LDEGGYYISP RITFPSLQAL VQHYSKKGDG LCQRLTLPCV RPAPQNPWAQ DEWEIPRQSL RLVRKLGSGQ FGEVWMGYYK NNMKVAIKTL KEGTMSPEAF LGEANVMKAL QHERLVRLYA VVTKEPIYIV TEYMARGCLL DFLKTDEGSR LSLPRLIDMS AQIAEGMAYI ERMNSIHRDL RAANILVSEA LCCKIADFGL ARIIDSEYTA QEGAKFPIKW TAPEAIHFGV FTIKADVWSF GVLLMEVVTY GRVPYPGMSN PEVIRNLERG YRMPRPDTCP PELYRGVIAE CWRSRPEERP TFEFLQSVLE DFYTATERQY ELQPLEHHHH HH
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
- Discover our top product BLK Protein
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B Lymphoid Tyrosine Kinase (BLK) (AA 1-505) protein (GST tag)
BLK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
B Lymphoid Tyrosine Kinase (BLK) (AA 1-90) protein (GST tag)BLK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
B Lymphoid Tyrosine Kinase (BLK) (AA 1-505) protein (His tag)BLK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
B Lymphoid Tyrosine Kinase (BLK) (Active) protein (GST tag)BLK Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 88 % as determined by reducing SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μM filtered solution of 20 mM Tris, 500 mM NaCl, 1 mM DTT, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- BLK (B Lymphoid Tyrosine Kinase (BLK))
- Alternative Name
- BLK (BLK Products)
- Synonyms
- bltk Protein, si:dkey-33i22.2 Protein, zgc:136231 Protein, MODY11 Protein, BLK proto-oncogene, Src family tyrosine kinase Protein, B lymphoid kinase Protein, blk Protein, BLK Protein, Blk Protein
- Gene ID
- 640
- UniProt
- P51451
- Pathways
- Positive Regulation of Peptide Hormone Secretion, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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