HGF Protein (Transcript Variant 1)

Catalog No. ABIN5505653

Product Details

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: Hi-5 Cells

Application: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human Hepatocyte growth factor / HGF (transcript variant 1) protein expressed in Hi-5 insect cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Resuspend the protein in the desired concentration in proper buffer

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Alternative Name: Hepatocyte growth factor / HGF (HGF Products)

Synonyms: DFNB39 Protein, F-TCF Protein, HGFB Protein, HPTA Protein, SF Protein, HGF Protein, C230052L06Rik Protein, HGF/SF Protein, NK1 Protein, NK2 Protein, SF/HGF Protein, hepatocyte growth factor Protein, HGF Protein, Hgf Protein

Background: This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss.

Molecular Weight: 83.13

NCBI Accession: NP_000592

Pathways: RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor