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CD14 Protein (CD14) (His tag)

CD14 Origin: Human Host: CHO Cells Recombinant >95 % by SDS-PAGE WB, ELISA, Agon, DB
Catalog No. ABIN5663349
  • Target See all CD14 Proteins
    CD14
    Protein Type
    Recombinant
    Origin
    • 18
    • 4
    • 4
    • 1
    • 1
    • 1
    Human
    Source
    • 15
    • 9
    • 2
    • 1
    • 1
    CHO Cells
    Purification tag / Conjugate
    This CD14 protein is labelled with His tag.
    Application
    Western Blotting (WB), ELISA, Agonist (Agon), Dot Blot (DB)
    Sequence
    MERASCLLLL LLPLVHVSAT TPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV PACARSTLSV G VSGTLVLLQG ARGFA
    Characteristics
    The hCD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.
    Purification
    affinity chromatography with monoclonal CD14 antibody biG2
    Purity
    >95 % by SDS-PAGE
    Endotoxin Level
    <1EU/μg of recombinant human CD14 as determined by LAL method
    Biological Activity Comment
    Up to 20 μg/ml CD14 inhibit binding of FITC-LPS (0.5μg/ml) to 6 x 105 CD14+CHO transfectants (FACS)
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    Discover our top product CD14 Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute with 40 μL sterile distilled water to a concentration of 0.25 mg/mL
    Concentration
    0.25 mg/mL
    Buffer
    sterile-filtered phosphate balanced salt solution, pH 7.2
    Preservative
    Without preservative
    Storage
    RT,-20 °C,-80 °C
    Storage Comment

    Storage: -20°C or -70°C

    Storage/Stability: Long time storage at -70oC. When stored at recommended temperature, this protein is stable for 12 months. The lyophilized product thought stable room temperature up to 3 weeks, but is best stored desiccated at -70oC more than 1 year, at refrigerator about 10 months. Reconstituted protein should be used immediately or stored in undiluted working aliquots at -20oC. For long-term storage it is recommended to add a carrier protein (o.1% BSA). Repeated freezing and thawing should be avoided !!!

    Expiry Date
    12 months
  • Target
    CD14
    Alternative Name
    CD14 (CD14 Products)
    Synonyms
    CD14 molecule Protein, CD14 antigen Protein, CD14 Protein, Cd14 Protein
    Background
    Background: CD14 acts as a receptor for endotoxin (LPS), expressed strongly on monocytes, macrophages and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor LPS-LBP (lipopolysaccharide binding protein)-complexes. CD14 cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.The rhuCD14 was produced in human CD14 transfected CHO-cells in serum free medium and has been purified by affinity chromatography with monoclonal antibody to hCD14 biG2at column and controlled by SDS page (90-95 % purity). Before transfection, the complete human CD14-cDNA amplified by PCR and cloned into expression vector p-POL-DHFR. CD14 is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes, also expressed by most tissue macrophages.
    Molecular Weight
    ~50kDa
    Gene ID
    929
    NCBI Accession
    NP_000582
    UniProt
    P08571
    Pathways
    TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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