MUSK Protein (AA 24-495, Extracellular) (GST tag)

Catalog No. ABIN5709979

Product Details

Target: MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))

Protein Type: Recombinant

Protein Characteristics: Extracellular, AA 24-495

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MUSK protein is labelled with GST tag.

Application: SDS-PAGE (SDS)

Sequence: LPKAPVITTP LETVDALVEE VATFMCAVES YPQPEISWTR NKILIKLFDT RYSIRENGQL LTILSVEDSD DGIYCCTANN GVGGAVESCG ALQVKMKPKI TRPPINVKII EGLKAVLPCT TMGNPKPSVS WIKGDSPLRE NSRIAVLESG SLRIHNVQKE DAGQYRCVAK NSLGTAYSKV VKLEVEEESE PEQDTKVFAR ILRAPESHNV TFGSFVTLHC TATGIPVPTI TWIENGNAVS SGSIQESVKD RVIDSRLQLF ITKPGLYTCI ATNKHGEKFS TAKAAATISI AEWREYCLAV KELFCAKEWL VMEEKTHRGL YRSEMHLLSV PECSKLPSMH WDPTACARLP HLAFPPMTSS KPSVDIPNLP SSSSSSFSVS PTYSMTVIIS IMSSFAIFVL LTITTLYCCR RRKQWKNKKR ESAAVTLTTL PSELLLDRLH PNPMYQRMPL LLNPKLLSLE YPRNNIEYVR DI

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))

Alternative Name: MUSK (MUSK Products)

Synonyms: unp Protein, fj98e12 Protein, zgc:63467 Protein, wu:fj98e12 Protein, si:dz46m7.2 Protein, si:dz57f23.3 Protein, si:busm1-57f23.3 Protein, musk-A Protein, MuSK Protein, MUSK Protein, Mdk4 Protein, Mlk Protein, Nsk1 Protein, Nsk2 Protein, Nsk3 Protein, muscle Protein, muscle associated receptor tyrosine kinase Protein, muscle, skeletal, receptor tyrosine kinase Protein, muscle associated receptor tyrosine kinase S homeolog Protein, MUSK Protein, musk Protein, musk.S Protein, Musk Protein

Background: Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle . Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic mbrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to mbranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous syst by mediating cholinergic responses, synaptic plasticity and mory formation .1 Publication

Molecular Weight: 79.9 kDa

UniProt: O15146

Pathways: RTK Signaling, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development