KAP1 Protein (AA 22-291, partial) (GST tag)

Catalog No. ABIN5712399

Product Details

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Protein Type: Recombinant

Protein Characteristics: AA 22-291, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This KAP1 protein is labelled with GST tag.

Application: SDS-PAGE (SDS)

Sequence: PGEGSAGGEK RSTAPSAAAS ASASAAASSP AGGGAEALEL LEHCGVCRER LRPEREPRLL PCLHSACSAC LGPAAPAAAN SSGDGGAAGD GTVVDCPVCK QQCFSKDIVE NYFMRDSGSK AATDAQDANQ CCTSCEDNAP ATSYCVECSE PLCETCVEAH QRVKYTKDHT VRSTGPAKSR DGERTVYCNV HKHEPLVLFC ESCDTLTCRD CQLNAHKDHQ YQFLEDAVRN QRKLLASLVK RLGDKHATLQ KSTKEVRSSI RQVSDVQKRV

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: TIF1B (TRIM28 Products)

Synonyms: KAP1 Protein, RNF96 Protein, TF1B Protein, TIF1B Protein, TRIM28 Protein, kap1 Protein, rnf96 Protein, tf1b Protein, tif1b Protein, AA408787 Protein, KAP-1 Protein, KRIP-1 Protein, Tif1b Protein, Tif1beta Protein, Kap1 Protein, Krip1 Protein, TIF1BETA Protein, tripartite motif containing 28 Protein, tripartite motif containing 28 L homeolog Protein, tripartite motif-containing 28 Protein, TRIM28 Protein, trim28.L Protein, Trim28 Protein

Background: Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome rodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation, the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg)

Molecular Weight: 56.1 kDa

UniProt: Q13263

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus