SIRPA Protein (AA 27-373) (His tag)
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- Target See all SIRPA Proteins
- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-373
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SIRPA protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSGVAGEEE LQVIQPDKSV LVAAGETATL RCTATSLIPV GPIQWFRGAG PGRELIYNQK EGHFPRVTTV SDLTKRNNMD FSIRIGNITP ADAGTYYCVK FRKGSPDDVE FKSGAGTELS VRAKPSAPVV SGPAARATPQ HTVSFTCESH GFSPRDITLK WFKNGNELSD FQTNVDPVGE SVSYSIHSTA KVVLTREDVH SQVICEVAHV TLQGDPLRGT ANLSETIRVP PTLEVTQQPV RAENQVNVTC QVRKFYPQRL QLTWLENGNV SRTETASTVT ENKDGTYNWM SWLLVNVSAH RDDVKLTCQV EHDGQPAVSK SHDLKVSAHP KEQGSNTAAE NTGSNERNIY
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product SIRPA Protein
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Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (His tag)
SIRPA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (Fc Tag)SIRPA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (His tag)SIRPA Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) protein (Fc-His Tag)SIRPA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Signal-Regulatory Protein alpha (SIRPA) protein (Fc-Avi Tag,Biotin)SIRPA Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 32-373) protein (His tag)SIRPA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) (Active) protein (His tag)SIRPA Origin: Cynomolgus Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (His tag)SIRPA Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (His tag)SIRPA Origin: Cynomolgus Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (Biotin,His-Avi Tag)SIRPA Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In PBS buffer ( pH 7.4) containing 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
- Alternative Name
- SIRPA (SIRPA Products)
- Synonyms
- BIT Protein, CD172A Protein, MFR Protein, MYD-1 Protein, P84 Protein, PTPNS1 Protein, SHPS1 Protein, SIRP Protein, SIRPA Protein, SIRP-A1 Protein, SIRP-B1 Protein, AI835480 Protein, Bit Protein, CD172a Protein, Ptpns1 Protein, SHP-1 Protein, SHPS-1 Protein, swc3 Protein, signal regulatory protein alpha Protein, myeloperoxidase Protein, signal-regulatory protein alpha Protein, tyrosine-protein phosphatase non-receptor type substrate 1 Protein, SIRPA Protein, MPO Protein, LOC458039 Protein, LOC100400378 Protein, LOC100590224 Protein, Sirpa Protein
- Background
- SIRPA is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. It was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. Recombinant human SIRPA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 40.4 kDa (370aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_542970
- UniProt
- P78324
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