NOG Protein (AA 28-144) (MBP tag,His tag)

Catalog No. ABIN6239823

Product Details

Target: NOG (Noggin (NOG))

Biological Activity: Active

Protein Characteristics: AA 28-144

Origin: Rat

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NOG protein is labelled with MBP tag,His tag.

Application: Cell Culture (CC), Activity Assay (AcA)

Characteristics: Tag location: Two N-terminal Tags, His-tag and MBP-tag

Purity: > 97 %

Biological Activity Comment: NOG (Noggin) is a signaling molecule that plays an important role in promoting somite patterning in the developing embryo, essential for cartilage morphogenesis and joint formation. It is considered as an inhibitor in bone morphogenetic proteins (BMP) signaling, by binding with BMP4, thus a binding ELISA assay was conducted to detect the association of recombinant rat NOG with BMP4. Briefly, NOG were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP4-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of NOG with BMP4 was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of NOG with BMP4.

Application Details

Application Notes: Isoelectric Point: 6.6

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: NOG (Noggin (NOG))

Abstract: NOG Products

Synonyms: SYM1 Protein, SYNS1 Protein, nog-A Protein, nog1 Protein, noggin-1 Protein, noggin Protein, noggin Protein, noggin L homeolog Protein, noggin protein Protein, NOG Protein, Nog Protein, nog.L Protein, noggin Protein

Background: Alternative Names: SYM1, SYNS1, Synostoses(Multiple)Syndrome 1, Symphalangism 1(Proximal)

Molecular Weight: 64kDa

UniProt: Q62809

Pathways: Stem Cell Maintenance, Tube Formation