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NOG Protein (AA 28-144) (MBP tag,His tag)

NOG Origin: Rat Host: Escherichia coli (E. coli) > 97 % CC, AcA Active
Catalog No. ABIN6239823
  • Target See all NOG Proteins
    NOG (Noggin (NOG))
    Biological Activity
    Active
    Protein Characteristics
    AA 28-144
    Origin
    • 12
    • 11
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    Rat
    Source
    • 17
    • 9
    • 4
    • 3
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This NOG protein is labelled with MBP tag,His tag.
    Application
    Cell Culture (CC), Activity Assay (AcA)
    Characteristics
    Tag location: Two N-terminal Tags, His-tag and MBP-tag
    Purity
    > 97 %
    Biological Activity Comment
    NOG (Noggin) is a signaling molecule that plays an important role in promoting somite patterning in the developing embryo, essential for cartilage morphogenesis and joint formation. It is considered as an inhibitor in bone morphogenetic proteins (BMP) signaling, by binding with BMP4, thus a binding ELISA assay was conducted to detect the association of recombinant rat NOG with BMP4. Briefly, NOG were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP4-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of NOG with BMP4 was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of NOG with BMP4.
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  • Application Notes
    Isoelectric Point: 6.6
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
    Preservative
    Dithiothreitol (DTT), ProClin
    Precaution of Use
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    NOG (Noggin (NOG))
    Abstract
    NOG Products
    Synonyms
    SYM1 Protein, SYNS1 Protein, nog-A Protein, nog1 Protein, noggin-1 Protein, noggin Protein, noggin Protein, noggin L homeolog Protein, noggin protein Protein, NOG Protein, Nog Protein, nog.L Protein, noggin Protein
    Background
    Alternative Names: SYM1, SYNS1, Synostoses(Multiple)Syndrome 1, Symphalangism 1(Proximal)
    Molecular Weight
    64kDa
    UniProt
    Q62809
    Pathways
    Stem Cell Maintenance, Tube Formation
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