Liver Arginase Protein (active Mutant)

Catalog No. ABIN6253489

Product Details

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Protein Type: Recombinant

Protein Characteristics: active Mutant

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Arginase I (human) (rec.) (highly active)

Specificity: Full length human arginase I.

Characteristics: Protein. Full length human arginase I. Source: E. coli. Liquid. In 10  mM TRIS-HCl,  pH 7.5, containing 1  mM beta-mercaptoethanol, 1  mM MnCl2 and 50 % glycerol. Purity: >90 % (SDS-PAGE). Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Arginase is involved in the nitric oxide (NO) pathway and immune cell arginine metabolism. It is fundamentally involved in cancer, inflammation, infections, fibrotic diseases, neurobiology, pregnancy and immune regulation in general.

Purity: >90 % (SDS-PAGE)

Biological Activity Comment: 1.6 ±0.2U/μg protein. One unit is defined as the amount of enzyme that converts 1μmol of L-arginine to L-ornithine and urea per min. at 37°C, pH 9.5 (according to protocol from R.T. Schimke, et al.; J. Biol. Chem. 238, 1012 (1963)).

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. In 10  mM TRIS-HCl,  pH 7.5, containing 1  mM beta-mercaptoethanol, 1  mM MnCl2 and 50 % glycerol.

Handling Advice: Avoid freeze/thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 1 year after receipt when stored at -80°C.

Target Details

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: Arginase I (ARG1 Products)

Synonyms: SI:zC146F4.4 (novel protein with NUDIX domain) Protein, si:ch211-146f4.3 Protein, argi1 Protein, AI Protein, AI256583 Protein, Arg-1 Protein, PGIF Protein, arginase 1 Protein, arginase Protein, Arginase-1 Protein, arginase, liver Protein, L-arginase Protein, arg1 Protein, PGTG_16455 Protein, argi1 Protein, ARG1 Protein, Arg1 Protein

Background:

Alternate Names/Synonyms: EC 3.5.3.1, ARG1, Arginase 1, Type I Arginase, Liver-type Arginase, L-Arginase

Product Description: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Arginase is involved in the nitric oxide (NO) pathway and immune cell arginine metabolism. It is fundamentally involved in cancer, inflammation, infections, fibrotic diseases, neurobiology, pregnancy and immune regulation in general.

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin