Liver Arginase Protein (Myc-DYKDDDDK Tag)
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- Target See all Liver Arginase (ARG1) Proteins
- Liver Arginase (ARG1) (Arginase, Liver (ARG1))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Liver Arginase protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human Arginase-1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- Arginase activity verified in a biochemical assay: Arginase 1 (ARG1, ) activity was measured in a colorimetric biochemical assay. Arginase 1 catalyzes the conversion of arginine to ornithine and urea. After incubation of the protein in a solution containing L-arginine, the reaction is stopped, and the urea concentration is measured by a chemical reaction that produces a colored product that absorbs at 430 nm. By measuring the absorbance at 430 nm and comparing to a standard, the specific activity of this preparation of ARG1 was calculated to be approximately 10U/mg. Unit definition: 1 unit of ARG1 converts 1 μmole of L-arginine to ornithine and urea per minute at pH 9.5 and 37°C.
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Arginase, Liver (ARG1) (AA 1-322) protein (GST tag)
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Arginase, Liver (ARG1) (AA 1-323) protein (Strep Tag)Crystallography grade ARG1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- > 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Liver Arginase (ARG1) (Arginase, Liver (ARG1))
- Alternative Name
- Arginase-1 (ARG1 Products)
- Synonyms
- SI:zC146F4.4 (novel protein with NUDIX domain) Protein, si:ch211-146f4.3 Protein, argi1 Protein, AI Protein, AI256583 Protein, Arg-1 Protein, PGIF Protein, arginase 1 Protein, arginase Protein, Arginase-1 Protein, arginase, liver Protein, L-arginase Protein, arg1 Protein, PGTG_16455 Protein, argi1 Protein, ARG1 Protein, Arg1 Protein
- Background
- Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 34.6 kDa
- NCBI Accession
- NP_000036
- Pathways
- Cellular Response to Molecule of Bacterial Origin
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