EPOR Protein (AA 25-250) (His tag)
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- Target See all EPOR Proteins
- EPOR (Erythropoietin Receptor (EPOR))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 25-250
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This EPOR protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP GNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVL LDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRT RYTFAVRARM AEPSFGGFWS AWSEPVSLLT PSDLDPHHHH HH
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Measured by its ability to inhibit EPO dependent proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect less or equal to 70ng/ml.
- Top Product
- Discover our top product EPOR Protein
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Erythropoietin Receptor (EPOR) (AA 25-138) protein (His-IF2DI Tag)
EPOR Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Erythropoietin Receptor (EPOR) (AA 1-508) protein (GST tag)EPOR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Erythropoietin Receptor (EPOR) (AA 31-130) protein (GST tag)EPOR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Erythropoietin Receptor (EPOR) (Active) protein (His tag)EPOR Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- EPOR (Erythropoietin Receptor (EPOR))
- Alternative Name
- EPOR (EPOR Products)
- Synonyms
- EPOR Protein, epor Protein, EPO-R Protein, erythropoietin receptor Protein, erythropoietin receptor L homeolog Protein, EPOR Protein, epor Protein, Epor Protein, epor.L Protein
- Background
- EPOR, also known as Erythropoietin receptor, mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, it dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. So it may also activate the LYN tyrosine kinase. Recombinant human EPOR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 25.6kDa (232aa) 28-40kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_000112
- UniProt
- P19235
- Pathways
- JAK-STAT Signaling
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