CD25 Protein (His tag)

Catalog No. ABIN6964077

Product Details

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD25 protein is labelled with His tag.

Application: ELISA

Purpose: Recombinant human IL2RA Protein with C-terminal 6xHis tag

Specificity: IL2RA (Glu22-Cys213) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: affinity purification

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with deionized water

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Preservative: Without preservative

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: IL2RA (IL2RA Products)

Synonyms: CD25 Protein, IDDM10 Protein, IL2R Protein, TCGFR Protein, IL2RAC Protein, IL-2RA Protein, Il2r Protein, Ly-43 Protein, interleukin 2 receptor subunit alpha Protein, interleukin-2 receptor subunit alpha-like Protein, interleukin 2 receptor, alpha chain Protein, IL2RA Protein, Il2ra Protein, LOC100222957 Protein

Background: Synonymes: IL2RA,CD25,p55,IL2-RA,IL-2-RA
Description: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

Molecular Weight: predicted molecular mass of 22.6 kDa after removal of the signal peptide.The apparent molecular mass of IL2RA-His is 35-55 kDa due to glycosylation.

Gene ID: 3559

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation