CHEK1 Protein (AA 1-476) (His-GST)

Catalog No. ABIN7320012

This Recombinant CHEK1 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for CHEK1 Protein (AA 1-476) (His-GST) (ABIN7320012)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-476

Purification tag / Conjugate: This CHEK1 protein is labelled with His-GST.

Purpose: Recombinant Mouse CHK1/CHEK1 Protein (His & GST Tag)

Sequence: Met1-Thr476

Characteristics: A DNA sequence encoding the mouse CHEK1 (O35280-1) (Met1-Thr476) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Kinase activity untested

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 3 mM DTT, 10 % glycerol

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHK1/CHEK1

Background: C85740,Chk1,rad27,CHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.

Molecular Weight:

Calculated MW: 82.2 kDa

Observed MW: 78 kDa

UniProt: O35280

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair