CHEK1 Protein (GST tag)

Catalog No. ABIN7317105

Product Details

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This CHEK1 protein is labelled with GST tag.

Purpose: Recombinant Human CHK1/CHEK1 Protein (GST Tag)(Active)

Sequence: Met 1-Thr476

Characteristics: A DNA sequence encoding the human CHK1 (AAM78553.1) (Met1-Thr476) was fused with the GST tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 32 nmol/min/mg using CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 2 mM GSH

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHK1/CHEK1 (CHEK1 Products)

Synonyms: An08g10320 Protein, AO090003000441 Protein, CHK1 Protein, chk1 Protein, C85740 Protein, Chk1 Protein, rad27 Protein, id:ibd2720 Protein, zgc:56093 Protein, CHEK1 Protein, checkpoint kinase 1 Protein, serine/threonine-protein kinase chk1 Protein, serine/threonine-protein kinase Chk1 Protein, CAMK/CAMKL/CHK1 protein kinase Chk1 Protein, checkpoint kinase 1 S homeolog Protein, CHEK1 Protein, ANI_1_2436074 Protein, AOR_1_768154 Protein, PTRG_04183 Protein, SJAG_01680 Protein, PAAG_04978 Protein, MCYG_03290 Protein, VDBG_03742 Protein, chek1.S Protein, Chek1 Protein, chek1 Protein

Background:

Background: CHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: CHK1

Molecular Weight: 80.7kDa

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair