CHEK1 Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)
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- Target See all CHEK1 Proteins
- CHEK1 (Checkpoint Kinase 1 (CHEK1))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 3
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CHEK1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human CHK1 (transcript variant 3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product CHEK1 Protein
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Checkpoint Kinase 1 (CHEK1) (AA 1-476) (Active) protein (His-GST)
CHEK1 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
Checkpoint Kinase 1 (CHEK1) (AA 361-476) protein (GST tag)CHEK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Checkpoint Kinase 1 (CHEK1) protein (GST tag,His tag)CHEK1 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
Checkpoint Kinase 1 (CHEK1) (Active) protein (GST tag)CHEK1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
Checkpoint Kinase 1 (CHEK1) (AA 1-476) protein (Strep Tag)Crystallography grade CHEK1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Checkpoint Kinase 1 (CHEK1) (AA 1-476) protein (Strep Tag)Crystallography grade CHEK1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- CHEK1 (Checkpoint Kinase 1 (CHEK1))
- Alternative Name
- Chk1 (CHEK1 Products)
- Synonyms
- An08g10320 Protein, AO090003000441 Protein, CHK1 Protein, chk1 Protein, C85740 Protein, Chk1 Protein, rad27 Protein, id:ibd2720 Protein, zgc:56093 Protein, CHEK1 Protein, checkpoint kinase 1 Protein, serine/threonine-protein kinase chk1 Protein, serine/threonine-protein kinase Chk1 Protein, CAMK/CAMKL/CHK1 protein kinase Chk1 Protein, checkpoint kinase 1 S homeolog Protein, CHEK1 Protein, ANI_1_2436074 Protein, AOR_1_768154 Protein, PTRG_04183 Protein, SJAG_01680 Protein, PAAG_04978 Protein, MCYG_03290 Protein, VDBG_03742 Protein, chek1.S Protein, Chek1 Protein, chek1 Protein
- Background
- The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
- Molecular Weight
- 54.3 kDa
- NCBI Accession
- NP_001107594
- Pathways
- p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair
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