CDK2 Protein (His tag)

Catalog No. ABIN7319357

Product Details

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CDK2 protein is labelled with His tag.

Purpose: Recombinant Human CDK2 Protein (E.coli, His Tag)

Sequence: Met 1-Leu298

Characteristics: Recombinant Human Cyclin-Dependent Kinase 2 is produced by our E.coli expression system and the target gene encoding Met1-Leu298 is expressed with a 6His tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 200 mM NaCl, 1 mM DTT, 40 % Glycerol, pH 8.0.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: CDK2 (CDK2 Products)

Synonyms: p33(CDK2) Protein, A630093N05Rik Protein, wu:fa10c02 Protein, zgc:56598 Protein, zgc:77684 Protein, eg1 Protein, CDKN2 Protein, cdk2L Protein, cyclin dependent kinase 2 Protein, cyclin-dependent kinase 2 Protein, cyclin-dependent kinase 2 S homeolog Protein, CDK2 Protein, Cdk2 Protein, cdk2 Protein, cdk2.S Protein

Background:

Background: Cyclin-dependent kinase 2 (CDK2) belongs to the cyclin-dependent kinase of Ser/Thr protein kinase. CDK2 acts as a catalytic subunit of the cyclin dependent kinase complex, whose activity is restricted to the G1-S phage of the cell cycle, it is essential for the G1/S transition. The kinase activity of CDK2 can be regulated by the association with a cyclin subunit, its phosphorylation state and CDK inhibitors. The activation of the CDK2/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Try14 and Tyr15. The inhibition of CDK2-cyclin complex can also be attributed to association with p27Kip1 and p21Waf1/Cip1. The activation of CDK2 has been shown to be necessary for apoptosis of quiescent cells, such as neurons, thymocytes and endothelial cells.

Synonym: Cyclin-Dependent Kinase 2, Cell Division Protein Kinase 2, p33 Protein Kinase, CDK2, CDKN2

Molecular Weight: 36.1 kDa

NCBI Accession: NP_001789

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA