EPH Receptor A2 Protein (EPHA2) (Fc Tag)

Catalog No. ABIN7318432

Product Details

Target: EPH Receptor A2 (EPHA2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This EPH Receptor A2 protein is labelled with Fc Tag.

Purpose: Recombinant Human EphA2 Protein (Fc Tag)(Active)

Sequence: Ala24-Asn534

Characteristics: Recombinant Human Ephrin A Receptor 2 is produced by our Mammalian expression system and the target gene encoding Ala24-Asn534 is expressed with a Fc tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human Ephrin-A1-His(Cat: PKSH032388) at 1.5μg/ml(100 μl/well) can bind Human EphA2-Fc. The ED50 of Human EphA2-Fc is 12.43 ug/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: EPH Receptor A2 (EPHA2)

Alternative Name: EphA2 (EPHA2 Products)

Synonyms: ARCC2 Protein, CTPA Protein, CTPP1 Protein, CTRCT6 Protein, ECK Protein, epha2 Protein, MGC89505 Protein, AW545284 Protein, Eck Protein, Myk2 Protein, Sek-2 Protein, Sek2 Protein, id:ibd5152 Protein, rtk6 Protein, G42 Protein, XE10 Protein, epha2-A Protein, EPH receptor A2 Protein, Eph receptor A2 Protein, epoxide hydrolase Protein, eph receptor A2 a Protein, EPH receptor A2 L homeolog Protein, EPHA2 Protein, Epha2 Protein, epha2 Protein, ephA2 Protein, epha2a Protein, epha2.L Protein

Background:

Background: Ephrin type-A receptor 2/EphA2 is a member of the Eph receptor tyrosine kinase family which binds Ephrins A1, 2, 3, 4, and 5. A and B class Eph proteins have a common structural organization. Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. EphA2 becomes autophosphorylated following ligand binding and then interacts with SH2 domain-containing PI3-kinase to activate MAPK pathways. Reverse signaling is also propagated through the Ephrin ligand. Transcription of EphA2 is dependent on the expression of E-Cadherin, and can be induced by p53 family transcription factors. EphA2 is upregulated in breast, prostate, and colon cancer vascular endothelium. Its ligand, EphrinA1, is expressed by the local tumor cells. In some cases, EphA2 and EphrinA1 are expressed on the same blood vessels. EphA2 signaling cooperates with VEGF receptor signaling in promoting endothelial cell migration.

Synonym: Ephrin type-A receptor 2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, EPHA2,ARCC2,CTPA,CTPP1,CTRCT6,ECK

Molecular Weight: 83.0 kDa

UniProt: P29317

Pathways: RTK Signaling