EPH Receptor A4 Protein (EPHA4) (His tag)

Catalog No. ABIN7195529

Recombinant EPH Receptor A4 protein expressed in HEK-293 Cells.

Quick Overview for EPH Receptor A4 Protein (EPHA4) (His tag) (ABIN7195529)

Target: EPH Receptor A4 (EPHA4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purity: > 97 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This EPH Receptor A4 protein is labelled with His tag.

Purpose: Recombinant Rat EphA4 Protein (His Tag)(Active)

Sequence: Met1-Thr547

Characteristics: A DNA sequence encoding the rat EPHA4 (D3ZZK3) (Met1-Thr547) was expressed, fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized recombinant Rat EPHA4 Protein (His Tag) at 10 μg/mL (100 μl/well) can bind Recombinant rat EFNA5-Fc with a linear range of 7.8-12.5 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: EPH Receptor A4 (EPHA4)

Alternative Name: EphA4

Background:

Background: EPH receptor A4 (ephrin type-A receptor 4), also known as EphA4, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus, and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor−mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). The extracellular domain of an EphA4 interacts with ephrin ligands, which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.

Synonym: EPHA4

Molecular Weight: 59.8 kDa

UniProt: D3ZZK3

Pathways: RTK Signaling