KLRK1 Protein (His tag)

Catalog No. ABIN7319359

Product Details

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This KLRK1 protein is labelled with His tag.

Purpose: Recombinant Human NKG2D/CD314 Protein (His Tag)(Active)

Sequence: Phe78-Val216

Characteristics: Recombinant Human NKG2-D type II Integral Membrane Protein is produced by our Mammalian expression system and the target gene encoding Phe78-Val216 is expressed with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human NKG2D-His at 10μg/ml(100 μl/well) can bind Human MICA-Fc(Cat: PKSH032753). The ED50 of Human NKG2D-His is 19.8 ug/ml .

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Alternative Name: NKG2D/CD314 (KLRK1 Products)

Synonyms: KLRK1 Protein, NKG2D Protein, CD314 Protein, D12S2489E Protein, KLR Protein, NKG2-D Protein, D6H12S2489E Protein, Nkg2d Protein, Nkrp2 Protein, killer cell lectin like receptor K1 Protein, killer cell lectin-like receptor subfamily K, member 1 Protein, NKG2D protein Protein, KLRK1 Protein, Klrk1 Protein, NKG2D Protein

Background:

Background: NKG2-D type II integral membrane protein (NKG2D) is a type II transmembrane glycoprotein which belongs to the CD94/NKG2 family. NKG2D is expressed on natural killer (NK) cells, CD8+ alpha-beta and gamma-delta T-cells. As an activating and costimulatory receptor, it involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. It provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. It stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. NKG2D participates in NK cell-mediated bone marrow graft rejection and survival of NK cells. It Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.

Synonym: CD314, KLRK1,CD314 antigen,Killer cell lectin-like receptor subfamily K member 1, killer cell lectin-like receptor subfamily K, member 1, KLR, NK cell receptor D, NKG2-D, NKG2-D type II integral membrane protein, NKG2-D-activating NK recepto

Molecular Weight: 16.9 kDa

UniProt: P26718

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process