MAPKAP Kinase 5 Protein (GST tag,His tag)

Catalog No. ABIN7317049

Product Details

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This MAPKAP Kinase 5 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human MAPKAPK5 Protein (His & GST Tag)

Sequence: Met 1-Gln 471

Characteristics: A DNA sequence encoding the human MAPKAPK5 isoform 1 (NP_003659.2) (Met 1-Gln 471) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 76 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Alternative Name: MAPKAPK5 (MAPKAPK5 Products)

Synonyms: MAPKAPK5 Protein, MK5 Protein, PRAK Protein, MAPKAP-K5 Protein, MK-5 Protein, mitogen-activated protein kinase-activated protein kinase 5 Protein, MAP kinase-activated protein kinase 5 Protein, MAPKAPK5 Protein, mapkapk5 Protein, Mapkapk5 Protein

Background:

Background: MAPKAPK5 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family. MAPKAPK5 has significant sequence homology to mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK). It is widely distributed. MAPKAPK5 can be phosphorylated by extracellular-regulated kinase (ERK), and p38 kinase but not by c-jun N-terminal kinase (JNK)in vitro.Recombinant GST-MAPKAPK5 protein can phosphorylate a peptide derived from the regulatory light chain of myosin II. Phosphorylation of MAPKAPK5 by ERK and p38 kinase increased its activity by 9 and 15 fold respectively. Taken together, these data suggest that MAPKAPK5 is a novelin vitrosubstrate for ERK and p38 kinase. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 also mediates stress-induced small heat shock protein 27 phosphorylation.

Synonym: MAPKAP-K5,MK-5,MK5,PRAK

Molecular Weight: 82 kDa

NCBI Accession: NP_003659

Pathways: MAPK Signaling