AKT3 Protein
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- Target See all AKT3 Proteins
- AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- Baculovirus infected Insect Cells
- Purpose
- Recombinant Mouse AKT3 Protein (aa 106-479)(Active)
- Sequence
- Ala106-Glu479
- Characteristics
- A DNA sequence encoding the AKT3 (Q9WUA6-1) (Ala106-Glu479) was expressed and purified.
- Purity
- > 95 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 20 nmol/min/mg using synthetic GSK3-derived peptide (R11-SGRARTSSFAEPGGK) as substrate.
- Top Product
- Discover our top product AKT3 Protein
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V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) protein
AKT3 Origin: Human Host: Baculovirus infected Insect Cells Recombinant WB
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 106-479) (Active) protein (His-GST)AKT3 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 1-479) protein (GST tag)AKT3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 100-189) protein (GST tag)AKT3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 106-479) protein (GST tag,His tag)AKT3 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 2-479) protein (His tag)Crystallography grade AKT3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 2-479) protein (His tag)Crystallography grade AKT3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)AKT3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, STD, PI Active
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
- Alternative Name
- AKT3 (AKT3 Products)
- Synonyms
- MPPH Protein, PKB-GAMMA Protein, PKBG Protein, PRKBG Protein, RAC-PK-gamma Protein, RAC-gamma Protein, STK-2 Protein, AI851531 Protein, D930002M15Rik Protein, Nmf350 Protein, Pkbg Protein, AKT serine/threonine kinase 3 Protein, thymoma viral proto-oncogene 3 Protein, v-akt murine thymoma viral oncogene homolog 3b Protein, AKT3 Protein, Akt3 Protein, akt3b Protein
- Background
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Background: v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.
Synonym: AI851531,D930002M15Rik,Nmf350
- Molecular Weight
- 43.4 kDa
- Pathways
- PI3K-Akt Signaling, RTK Signaling, TLR Signaling, Hepatitis C, VEGF Signaling
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