AXL Protein (AA 33-449) (His tag)

Catalog No. ABIN7273880

Product Details

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Protein Type: Recombinant

Protein Characteristics: AA 33-449

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This AXL protein is labelled with His tag.

Purpose: Cynomolgus AXL Protein

Sequence: Glu33-Pro449

Characteristics: Recombinant Cynomolgus AXL Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu33-Pro449.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus AXL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA. The affinity constant of 1.70 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Alternative Name: AXL (AXL Products)

Synonyms: JTK11 Protein, UFO Protein, AI323647 Protein, Ark Protein, Tyro7 Protein, Ufo Protein, AXL receptor tyrosine kinase Protein, Axl receptor tyrosine kinase Protein, AXL Protein, Axl Protein

Background: Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.

Molecular Weight: 46.3 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Tris-Bis PAGE result.

UniProt: A0A1D5Q330

Pathways: RTK Signaling, Cellular Response to Molecule of Bacterial Origin