AXL Protein (His-Avi Tag)

Catalog No. ABIN7273884

Product Details

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This AXL protein is labelled with His-Avi Tag.

Sequence: Ala26-Pro449

Purity: > 95% as determined by Tris-Bis PAGE,> 95% as determined by SEC-HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human AXL, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Storage: 4 °C,-80 °C

Storage Comment: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Alternative Name: AXL (AXL Products)

Synonyms: JTK11 Protein, UFO Protein, AI323647 Protein, Ark Protein, Tyro7 Protein, Ufo Protein, AXL receptor tyrosine kinase Protein, Axl receptor tyrosine kinase Protein, AXL Protein, Axl Protein

Background: Axl, UFO, AXL oncogene, AXL, ARK, JTK11, Tyro7, AI323647, EC 2.7.10, EC 2.7.10.1,Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.

Molecular Weight: 48.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Pathways: RTK Signaling, Cellular Response to Molecule of Bacterial Origin