IL2 Receptor beta Protein (His-Avi Tag,Biotin)

Catalog No. ABIN7274077

Product Details

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL2 Receptor beta protein is labelled with His-Avi Tag,Biotin.

Sequence: Ala27-Asp239

Purity: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.101 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Storage: 4 °C,-80 °C

Storage Comment: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Alternative Name: IL-2 R beta (IL2RB Products)

Synonyms: IL2RB Protein, il-2rb Protein, CD122 Protein, IL15RB Protein, P70-75 Protein, IL2RBC Protein, IL-15Rbeta Protein, IL15Rbeta Protein, Il-2/15Rbeta Protein, Il-2Rbeta Protein, p70 Protein, interleukin 2 receptor subunit beta Protein, interleukin-2 receptor subunit beta Protein, interleukin 2 receptor, beta Protein, interleukin 2 receptor, beta chain Protein, IL2RB Protein, LOC510185 Protein, il2rb Protein, Il2rb Protein

Background: CD122, IL-15 R beta, IL-2 R beta, IL-2R subunit beta, IL2RB, P70-75, p75, RP5-1170K4.6,IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains.

Molecular Weight: 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

NCBI Accession: NP_000869

Pathways: JAK-STAT Signaling, Growth Factor Binding