CD3D & CD3E (AA 22-117) protein (Fc Tag)

Catalog No. ABIN7274130

Product Details

Target: CD3D & CD3E

Protein Type: Recombinant

Protein Characteristics: AA 22-117

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: Fc Tag

Purpose: Cynomolgus CD3E & CD3D/CD3 epsilon & CD3 delta Protein

Sequence: Gln22-Asp117 (CD3E) & Phe22-Ala105 (CD3D)

Specificity: Uni-Prot: Q95LI5.2 (CD3E), Q95LI8 (CD3D)

Characteristics: Recombinant Cynomolgus CD3E & CD3D/CD3 epsilon & CD3 delta Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus CD3E&CD3D, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Antibody, mFc Tag with EC50 of 13.9ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD3D & CD3E

Alternative Name: CD3E&CD3D

Synonyms: AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, CD3 antigen, epsilon polypeptide Protein, Cd3e Protein

Background: T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Molecular Weight: 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 58-62 kDa (CD3E) and 44-46 kDa (CD3D) based on Tris-Bis PAGE result.

UniProt: Q95LI5