CD3D & CD3E (AA 23-126) protein (Fc Tag)
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- Target
- CD3D & CD3E
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-126
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- Fc Tag
- Purpose
- Human CD3E & CD3D/CD3 epsilon & CD3 delta Protein
- Sequence
- Asp23-Asp126 (CD3E) & Phe22-Ala105 (CD3D)
- Specificity
- Uni-Prot: P07766 (CD3E), P04234 (CD3D)
- Characteristics
- Recombinant Human CD3E & CD3D/CD3 epsilon & CD3 delta Protein is expressed from HEK293 with hFc at the C-Terminus.It contains Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D).
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD3E&CD3D, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.68ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD3D & CD3E
- Alternative Name
- CD3E&CD3D
- Synonyms
- AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, CD3 antigen, epsilon polypeptide Protein, Cd3e Protein
- Background
- T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
- Molecular Weight
- 37.8 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
- UniProt
- P07766
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