CD3D & CD3E (AA 23-120) protein (His tag,Biotin)

Catalog No. ABIN7274137

Product Details

Target: CD3D & CD3E

Protein Type: Recombinant

Protein Characteristics: AA 23-120

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: His tag,Biotin

Purpose: Biotinylated Human CD3E & CD3D/CD3 epsilon & CD3 delta Protein (Primary Amine Labeling)

Sequence: Asp23-Glu120 (CD3E) & Phe22-Ala105 (CD3D)

Specificity: Uni-Prot: P07766 (CD3E), P04234 (CD3D)

Characteristics: Recombinant Biotinylated Human CD3E & CD3D/CD3 epsilon & CD3 delta Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CD3 Antibody, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, His Tag with the EC50 of 0.14μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD3D & CD3E

Alternative Name: CD3E&CD3D

Synonyms: AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, CD3 antigen, epsilon polypeptide Protein, Cd3e Protein

Background: T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Molecular Weight: 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.

UniProt: P07766