CD74 Protein (AA 73-232) (His tag)
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- Target See all CD74 Proteins
- CD74 (HLA-DR-gamma (CD74))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 73-232
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD74 protein is labelled with His tag.
- Purpose
- Human CD74/DHLAG Protein
- Sequence
- Gln73-Met232
- Characteristics
- Recombinant Human CD74/DHLAG Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln73-Met232.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD74 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. See testing image for detail.
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HLA-DR-gamma (CD74) (AA 56-279) protein (His tag)
CD74 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA-DR-gamma (CD74) (AA 1-160) protein (GST tag)CD74 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
HLA-DR-gamma (CD74) protein (His tag,mFc Tag)CD74 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
HLA-DR-gamma (CD74) (Active) protein (His tag)CD74 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >90 % as determined by reducing SDS-PAGE. Active
HLA-DR-gamma (CD74) (AA 73-232) protein (His tag)CD74 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
HLA-DR-gamma (CD74) (AA 73-296) protein (His tag)Crystallography grade CD74 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
HLA-DR-gamma (CD74) (AA 56-279) protein (His tag)Crystallography grade CD74 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
HLA-DR-gamma (CD74) (AA 1-296) protein (Strep Tag)Crystallography grade CD74 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
HLA-DR-gamma (CD74) (AA 1-279) protein (Strep Tag)Crystallography grade CD74 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD74 (HLA-DR-gamma (CD74))
- Alternative Name
- CD74 (CD74 Products)
- Synonyms
- DHLAG Protein, HLADG Protein, II Protein, Ia-GAMMA Protein, CLIP Protein, Ii Protein, INVG34 Protein, Iclp-1 Protein, iclp1 Protein, wu:fb11f09 Protein, wu:fb12a01 Protein, wu:fb13d06 Protein, wu:fk94e07 Protein, Iclp-2 Protein, cd74 Protein, iclp2 Protein, zgc:123161 Protein, CD74 molecule Protein, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) Protein, CD74 molecule, major histocompatibility complex, class II invariant chain a Protein, CD74 molecule, major histocompatibility complex, class II invariant chain b Protein, CD74 Protein, Cd74 Protein, cd74a Protein, cd74b Protein
- Background
- CD74 (invariant MHC class II) regulates protein trafficking and is a receptor for macrophage migration inhibitory factor (MIF) and d-dopachrome tautomerase (d-DT/MIF-2). CD74 expression is increased in tubular cells and/or glomerular podocytes and parietal cells in human metabolic nephropathies, polycystic kidney disease, graft rejection and kidney cancer and in experimental diabetic nephropathy and glomerulonephritis.
- Molecular Weight
- 19.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
- NCBI Accession
- NP_004346
- Pathways
- Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints
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