SARS-CoV-2 Spike S1 Protein (D614G) (His-Avi Tag)

Catalog No. ABIN7276479

Product Details

Target: SARS-CoV-2 Spike S1

Protein Type: Recombinant

Protein Characteristics: D614G

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Spike S1 protein is labelled with His-Avi Tag.

Purpose: SARS-COV-2 Spike S1 (D614G) Protein

Sequence: Gln14-Arg683 (D614G)

Characteristics: Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln14-Arg683(D614G).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2μg/ml (100μl/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 42ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike S1

Abstract: SARS-CoV-2 Spike S1 Products

Synonyms: E2 Protein, Surface Glycoprotein Protein, S Protein

Target Type: Viral Protein

Background: The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.

Molecular Weight: 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.