DLL3 Protein (AA 27-490) (His tag)
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- Target See all DLL3 Proteins
- DLL3 (delta Like Protein 3 (DLL3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-490
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Origin
- Rhesus Monkey
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DLL3 protein is labelled with His tag.
- Purpose
- Rhesus macaque DLL3 Protein
- Sequence
- Ala27-Arg490
- Characteristics
- Recombinant Rhesus macaque DLL3 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ala27-Arg490.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Rhesus macaque DLL3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. See testing image for detail.
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delta Like Protein 3 (DLL3) (AA 27-490) protein (His tag)
DLL3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
delta Like Protein 3 (DLL3) (AA 27-477) protein (His tag)DLL3 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE,> 90 % as determined by SEC-HPLC
delta Like Protein 3 (DLL3) (AA 25-488) protein (His tag)DLL3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
delta Like Protein 3 (DLL3) (AA 27-215) protein (His-DYKDDDDK Tag)DLL3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
delta Like Protein 3 (DLL3) (AA 27-490) protein (His tag,Biotin)DLL3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
delta Like Protein 3 (DLL3) (AA 27-492) protein (His tag)DLL3 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
delta Like Protein 3 (DLL3) (AA 27-490) (Active) protein (His tag)DLL3 Origin: Cynomolgus Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
delta Like Protein 3 (DLL3) (AA 27-492) (Active) protein (His tag,FITC)DLL3 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM Tris, 50 mM NaCl, 200 mM L-arginine ( pH 8.5). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- DLL3 (delta Like Protein 3 (DLL3))
- Alternative Name
- DLL3 (DLL3 Products)
- Synonyms
- SCDO1 Protein, pu Protein, pudgy Protein, delta like canonical Notch ligand 3 Protein, delta-like 3 (Drosophila) Protein, DLL3 Protein, Dll3 Protein
- Background
- Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).
- Molecular Weight
- 51 kDa. Due to glycosylation,the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
- UniProt
- F6TFV4
- Pathways
- Notch Signaling
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